Microneurography in diabetic mice
| Summary |
Data Summary |
| Investigator |
Baumann, Thomas |
| Description |
Spontaneous burning pain is a common symptom of diabetic peripheral neuropathy
in humans. Yet there are few objective measures and no clear surrogate marker
for spontaneous pain in laboratory animals. The conventional
electrophysiological test currently employed by the consortium (nerve conduction
velocity measurement) is incapable of providing information regarding burning
pain because this test measures only the amplitude and latency of the compound
action potential produced by the large diameter (A脽) primary afferent fibers
which supply low-threshold mechanoreceptors. Unlike the small-diameter (C)
nociceptive fibers, A脽 fibers do not contribute to the burning pain of diabetic
neuropathy. Small fiber neuropathy can exist in the absence of large fiber
neuropathy. Behavioral assays (measurement of withdrawal latencies in response
to noxious thermal stimulation) provide only an indirect measure of diabetic
neuropathy. Histologic assays (measurement of nerve fiber density in the skin)
cannot substitute for direct measurements of nerve fiber discharge. For these
reasons we propose to add microneurography to the arsenal employed for
phenotyping diabetic mice. Microneurography has been used for decades in studies
of the neural substrate of human somatosensory perception. There is a wealth of
background information. Among other achievements, microneurographic studies in
humans demonstrated that spontaneous burning pain in patients with diabetic
neuropathy is associated with spontaneous action potential discharge in
nociceptive C-fiber primary afferents. Recently the microneurography technique
was adapted for recording C-fiber activity in rats. We propose to adapt this
technique for use in mice. Comparison of the patterns of C-fiber discharge
measured in diabetic humans who do or do not experience spontaneous burning pain
to the patterns of the C-fiber activity measured in different strains of
diabetic mice will ultimately permit the selection of the most represen
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| Status |
Completed |
| Public Release |
9/1/2010 |
| Data Collected? |
Data will not be collected for this catalog item |
| Species |
M. musculus |
| Animal Age |
Measured In: week(s) post-natal (w) |
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| Data Analysis |
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| Type | Count | Released |
| Animals | 0 | - |
| Experimental Conditions | 0 | - |
| Phenotype Assays | 0 | - |
| Phenotype Measurements | 0 | 0 |
| Microarrays | 0 | - |
| Histology Images | 0 | 0 |
| Publications | 0 | - |
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Experimental Factor Values