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Genomic analysis of metabolic pathway gene expression in mice.
Authors Ghazalpour A, Doss S, Sheth SS, Ingram-Drake LA, Schadt EE, Lusis AJ, Drake TA
Submitted By Willa Hsueh on 2/11/2009
Status Published
Journal Genome biology
Year 2005
Date Published 9/1/2005
Volume : Pages 6(7) : R59
PubMed Reference
Abstract BACKGROUND: A segregating population of (C57BL/6J x DBA/2J)F2 intercross mice
was studied for obesity-related traits and for global gene expression in liver.
Quantitative trait locus analyses were applied to the subcutaneous fat-mass
trait and all gene-expression data. These data were then used to identify gene
sets that are differentially perturbed in lean and obese mice. RESULTS: We
integrated global gene-expression data with phenotypic and genetic segregation
analyses to evaluate metabolic pathways associated with obesity. Using two
approaches we identified 13 metabolic pathways whose genes are coordinately
regulated in association with obesity. Four genomic regions on chromosomes 3, 6,
16, and 19 were found to control the coordinated expression of these pathways.
Using criteria that included trait correlation, differential gene expression,
and linkage to genomic regions, we identified novel genes potentially associated
with the identified pathways. CONCLUSION: This study demonstrates that genetic
and gene-expression data can be integrated to identify pathways associated with
clinical traits and their underlying genetic determinants.

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