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Methods for defining distinct bioenergetic profiles in platelets, lymphocytes,
monocytes, and neutrophils, and the oxidative burst from human blood.
Authors Chacko BK, Kramer PA, Ravi S, Johnson MS, Hardy RW, Ballinger SW, Darley-Usmar
VM
Submitted By Victor Darley-Usmar on 11/4/2014
Status Published
Journal Laboratory investigation; a journal of technical methods and pathology
Year 2013
Date Published 6/1/2013
Volume : Pages 93 : 690 - 700
PubMed Reference
Abstract Peripheral blood mononuclear cells and platelets have long been recognized as
having the potential to act as sensitive markers for mitochondrial dysfunction
in a broad range of pathological conditions. However, the bioenergetic function
of these cells has not been examined from the same donors, yet this is important
for the selection of cell types for translational studies. Here, we demonstrate
the measurement of cellular bioenergetics in isolated human monocytes,
lymphocytes, and platelets, including the oxidative burst from neutrophils and
monocytes from individual donors. With the exception of neutrophils, all cell
types tested exhibited oxygen consumption that could be ascribed to oxidative
phosphorylation with each having a distinct bioenergetic profile and
distribution of respiratory chain proteins. In marked contrast, neutrophils were
essentially unresponsive to mitochondrial respiratory inhibitors indicating that
they have a minimal requirement for oxidative phosphorylation. In monocytes and
neutrophils, we demonstrate the stimulation of the oxidative burst using phorbol
12-myristate 13-acetate and its validation in normal human subjects. Taken
together, these data suggest that selection of cell type from blood cells is
critical for assessing bioenergetic dysfunction and redox biology in
translational research.


Investigators with authorship
NameInstitution
Victor Darley-UsmarUniversity of Alabama at Birmingham

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