¿´Æ¬ÊÓƵ

Object moved to here.

¿´Æ¬ÊÓƵ :: Pilot & Feasibility Program Application

¿´Æ¬ÊÓƵ


Gene Expression in the Black Bear Kidney: A first Step Towards Understanding Recovery in Mammalian Kidneys
Summary Data Summary
Applicant Korstanje, Ron
E-Mail Address ron.korstanje@jax.org
Project Title Gene Expression in the Black Bear Kidney: A first Step Towards Understanding
Recovery in Mammalian Kidneys
CBU ID 15GHSU1472
External SubContract ID 25034-70
Diabetic Complication Nephropathy
Funding Program Group Pilot & Feasibility [PF2015]
Abstract The inability of the human kidney to recover from damage leads to an
ever-growing population of patients with chronic kidney disease that is
dependent on dialysis, with over 50% of patients due to diabetic nephropathy.
The broad goal of this study is to identify genetic and biological mechanisms
that could mediate nephron recovery, thereby enabling patients to avoid
dialysis. Evidence strongly suggests that kidney recovery takes place in the
American black bear, providing a potentially powerful and previously unexplored
platform for identifying genes and biological pathways that could regulate
kidney recovery in mammals, including humans. The American black bear becomes
hyperinsulinemic and develops characteristics of insulin resistance in the fall,
and during hibernation its renal function drops to a level comparable to a
dialysis patient. Furthermore, glomerulosclerosis, a hallmark of diabetic
nephropathy, has been reported in bears shortly after hibernation. Yet, bears
seem to recover within a short period of time after emerging from hibernation in
the spring, and do this year after year. However, little is known of the process
of kidney recovery in the black bear, including the time period of recovery and
the genes and biological pathways involved. In this pilot study we will carry
out the following Specific Aims: 1) determine a histological timeline of nephron
recovery in the black bear by collecting samples during the spring and fall, and
2) identify genes that are differentially expressed in black bear kidneys as a
function of nephron recovery through high-throughput sequencing of kidney RNA.
We have already set up a network with Maine guides and bear biologists from
Maine Native American tribes for collecting kidney samples by bear hunters and
have already collected 38 samples during the 2014 spring hunting season. In
addition, to enable gene expression analysis, we have completely sequenced the
black bear genome.
We expect to determine a reliable window during which nephron recovery in the
black bear takes place and identify a set of genes that shows an expression
pattern that follows the nephron recovery timeline. Results of these studies
will be the first insights into mammalian nephron recovery. They will establish
the foundation of future projects in which we will study the identified genes in
mouse models of diabetic nephropathy to understand their normal role and whether
manipulation of these genes or the molecular pathways in which they play a role,
will lead to recovery. Future work will translate the results into human
clinical studies.
Application PDF Application Research Plan
Status Contract Executed
Key Personnel
Salary Total Costs 33235
Supply Total Costs 4800
Equipment Total Costs 0
Travel/Other Total Costs 23466
Direct Costs 61501
Indirect Costs Proposed 12300
Total Costs Proposed 73801
Total Costs Approved 66421
Start Date 10/1/2015
End Date 9/30/2016
IFO Name Callagy, Kristen
IFO E-Mail Address kristen.callagy@jax.org
IACUC/IRB No. 99999
IACUC/IRB Institution Jackson Laboratory
Entity ID No. 010211513
Report Request Date 10/30/2016
T1D NO
TypeCount
Invoices 9
Progress Reports 1
Data Submission


Invoices
UrlCBU IDExternal IDInstitutionDateDirectIndirectInvoiceBalancePDF
  View  15GHSU147225034-70Jackson Laboratory9/30/2016$12,896.44$2,579.30$15,475.74$77.64View PDF
  View  15GHSU147225034-70Jackson Laboratory8/5/2016$10,762.73$2,116.74$12,879.47$77.64View PDF
  View  15GHSU147225034-70Jackson Laboratory7/11/2016$6,754.92$1,386.82$8,141.74$77.64View PDF
  View  15GHSU147225034-70Jackson Laboratory6/20/2016$5,419.99$1,084.00$6,503.99$77.64View PDF
  View  15GHSU147225034-70Jackson Laboratory5/11/2016$11,002.67$2,200.53$13,203.20$77.64View PDF
  View  15GHSU147225034-70Jackson Laboratory4/13/2016$865.65$173.13$1,038.78$77.64View PDF
  View  15GHSU147225034-70Jackson Laboratory3/14/2016$6,875.30$1,375.06$8,250.36$77.64View PDF
  View  15GHSU147225034-70Jackson Laboratory2/9/2016$195.40$39.08$234.48$77.64View PDF
  View  15GHSU147225034-70Jackson Laboratory1/25/2016$615.60-$615.60$77.64View PDF


Reports
Click here to cancel and return to funding program application

*Author:
*SubContract:
*Select File:

Click browse and select the file to upload.
(Please upload ONLY TXT, Image Files (not histolgy), PDF, XLS, XLSX, DOC, or DOCX files.)