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Role of Podocyte Lipids in the Prediction of Diabetic Nephropathy
Summary Data Summary
Applicant Fornoni, Alessia
E-Mail Address afornoni@med.miami.edu
Project Title Role of Podocyte Lipids in the Prediction of Diabetic Nephropathy
CBU ID 12GHSU176
External SubContract ID 25034-24
Diabetic Complication Nephropathy
Funding Program Group Pilot & Feasibility [PF2012]
Abstract Diabetic kidney disease (DKD) is responsible for nearly half of the incident
end-stage kidney disease (ESKD) in the USA. Early clinical diagnosis of DKD is
primarily based on measurement of urinary albumin-to-creatinine ratios (ACR),
but significant DKD lesions can be present in the absence of elevated ACR.
Several histological features are associated with progressive kidney disease and
correlate with DKD lesions in both type 1 and type 2 diabetes (T1D and T2D);
among them, podocyte foot process effacement and decreased podocyte number.
Intracellular lipid accumulation is described in glomeruli of rodent models of
DKD. Whether lipid accumulation in podocytes contributes to the progression of
DKD, however, remains to be established. Our preliminary data demonstrate that
normal cultured human podocytes exposed to the sera of patients with T1D and DKD
accumulate total and esterified cholesterol whereas those exposed to sera from
patients with T1D of similar duration and no DKD do not. Podocytes that
accumulate lipid exhibit significant actin remodeling and increased apoptosis.
This proposal will help us determine the role of intracellular lipid
accumulation in the initiation and progression of DKD. We hypothesize that
accumulation of intracellular lipid within podocytes precedes and predicts
clinically apparent DKD and that a minimally invasive in vitro bioassay can be
developed to predict DKD risk prior to increases in ACR in patients with T1D
(University of Minnesota cohort) and T2D (Pima Indian cohort). We propose the
following Specific Aims: 1. Develop a bioassay that predicts DKD using normal
cultured human podocytes exposed to the sera of diabetic patients. 2. Develop a
morphometric method to quantify lipid accumulation in podocytes.
Assays developed with this application will then be utilized for subsequent
larger interinstitutional and interdepartmental proposals to validate the
association between lipid accumulation in podocytes and the development and
progression of DKD in both T1D and T2D.
Application PDF Application Research Plan
Status Contract Executed
Key Personnel
Salary Total Costs 50130
Supply Total Costs 10779
Equipment Total Costs 0
Travel/Other Total Costs 30000
Direct Costs 90909
Indirect Costs Proposed 9091
Total Costs Proposed 100000
Total Costs Approved 100000
Start Date 5/1/2013
End Date 4/30/2014
IFO Name Garcia, Maria Amelia
IFO E-Mail Address MGarcia4@med.miami.edu
IACUC/IRB No. 99999
IACUC/IRB Institution University of Miami - Medical Campus
Entity ID No.
Report Request Date 4/30/2014
T1D NO
TypeCount
Invoices 9
Progress Reports 1
Data Submission


Invoices
UrlCBU IDExternal IDInstitutionDateDirectIndirectInvoiceBalancePDF
  View  12GHSU17625034-24University of Miami - Medical Campus6/24/2014$4,437.10-$4,437.10-View PDF
  View  12GHSU17625034-24University of Miami - Medical Campus4/9/2014$2,373.18-$2,373.18-View PDF
  View  12GHSU17625034-24University of Miami - Medical Campus3/25/2014$3,623.01$362.30$3,985.31-View PDF
  View  12GHSU17625034-24University of Miami - Medical Campus2/25/2015$6,544.66-$6,544.66-View PDF
  View  12GHSU17625034-24University of Miami - Medical Campus2/18/2014$2,157.44$215.74$2,373.18-View PDF
  View  12GHSU17625034-24University of Miami - Medical Campus12/13/2013$2,157.44$215.74$2,373.18-View PDF
  View  12GHSU17625034-24University of Miami - Medical Campus11/25/2013$5,036.55$503.66$5,540.21-View PDF
  View  12GHSU17625034-24University of Miami - Medical Campus1/22/2014$2,157.44$215.74$2,373.18-View PDF
  View  12GHSU17625034-24University of Miami - Medical Campus $70,000.00-$70,000.00- 


Reports
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