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Serial Block Face Scanning Electron Microscopy Studies of Diabetic Kidney Disease
Summary Data Summary
Applicant Najafian, Behzad
E-Mail Address najafian@uw.edu
Project Title Serial Block Face Scanning Electron Microscopy Studies of Diabetic Kidney
Disease
CBU ID 17AU3776
External SubContract ID 32307-3
Diabetic Complication Nephropathy
Funding Program Group Pilot & Feasibility [PF2017]
Abstract Diabetic kidney disease (DKD) is by far the most common cause of end stage
kidney disease in the US. Novel insights about the evolution of DKD lesions may
help identify new targets for treatment. Most of what we currently know about
the structural changes of DKD comes from the studies performed by
two-dimensional (2D) transmission electron microscopy (TEM); however, complexity
of some of the cellular and subcellular structures demands for 3D studies. Here,
we propose to apply serial block face scanning EM (SBF-SEM), a relatively novel
technique that allows for 3D EM studies, to study DKD in human and mice. We will
optimize SBFSEM tissue preparation protocols, will generate 3D models to better
understand the spatial relationships between the glomerular structures. Adding
quantitative approached to the volumetric data from SBF-SEM will make this much
more powerful. We will develop quantitative approaches to study mitochondrial
number and fission/fusion and subpodocyte space volume. Accomplishment of these
goals may help finding structural evidence of pathogenetic processes that may be
amenable to interventions. These could lead to proof of concept studies where
interventions altering these structural variables influence outcomes.
Application PDF Application Research Plan
Status Contract Executed
Key Personnel Alpers, Charles; Michael Mauer; Robert Nelson
Salary Total Costs 46192
Supply Total Costs 17002
Equipment Total Costs 0
Travel/Other Total Costs 0
Direct Costs 63194
Indirect Costs Proposed 36806
Total Costs Proposed 100000
Total Costs Approved 100000
Start Date 11/1/2017
End Date 10/31/2018
IFO Name DeFries, Kristen
IFO E-Mail Address gcahelp@uw.edu
IACUC/IRB No. 9999
IACUC/IRB Institution University of Washington
Entity ID No. 91-6001537
Report Request Date 11/30/2018
T1D NO
TypeCount
Invoices 15
Progress Reports 1
Data Submission


Invoices
UrlCBU IDExternal IDInstitutionDateDirectIndirectInvoiceBalancePDF
  View  17AU377632307-3University of Washington7/12/2018$2,631.20$1,447.16$4,078.36-View PDF
  View  17AU377632307-3University of Washington7/10/2019$648.85$344.64$993.49-View PDF
  View  17AU377632307-3University of Washington6/12/2019$4,821.82$2,652.00$7,473.82-View PDF
  View  17AU377632307-3University of Washington6/11/2018$2,627.44$1,445.09$4,072.53-View PDF
  View  17AU377632307-3University of Washington5/9/2019$2,349.77$1,292.38$3,642.15-View PDF
  View  17AU377632307-3University of Washington5/9/2018$3,552.82$1,954.05$5,506.87-View PDF
  View  17AU377632307-3University of Washington4/10/2019$11,919.18$1,958.71$13,877.89-View PDF
  View  17AU377632307-3University of Washington4/10/2018$10,382.05$5,710.12$16,092.17-View PDF
  View  17AU377632307-3University of Washington3/11/2019$3,174.01$1,738.17$4,912.18-View PDF
  View  17AU377632307-3University of Washington2/13/2019$2,740.07$1,507.04$4,247.11-View PDF
  View  17AU377632307-3University of Washington12/26/2019$5,517.78$2,333.45$7,851.23-View PDF
  View  17AU377632307-3University of Washington12/12/2018$2,504.54$1,377.51$3,882.05-View PDF
  View  17AU377632307-3University of Washington11/9/2018$3,622.57$1,992.41$5,614.98-View PDF
  View  17AU377632307-3University of Washington10/30/2018$8,960.30$4,928.16$13,888.46-View PDF
  View  17AU377632307-3University of Washington1/9/2019$2,494.65$1,372.06$3,866.71-View PDF
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