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Detecting diabetic neuropathy in rodents by imaging corneal nerves
Summary Data Summary
Applicant Calcutt, Nigel
E-Mail Address ncalcutt@ucsd.edu
Project Title Detecting diabetic neuropathy in rodents by imaging corneal nerves
CBU ID 09MCG92
External SubContract ID 23789-5
Diabetic Complication Neuropathy & Neurocognition
Funding Program Group Pilot & Feasibility [PF2009]
Abstract Visualization of corneal nerves by confocal microscopy is currently being
developed as a sensitive and completely non-invasive method of detecting
neuropathy in diabetic patients that allows the repeated measurements necessary
for longitudinal studies of drug efficacy. Corneal confocal microscopy (CCM) has
been applied to live rodents and other species and our exploratory studies
indicate that corneal nerves can also be visualized ex vivo. In the proposal we
will establish optimal conditions for visualizing and quantifying corneal nerves
in rats and mice in vivo and in eyes from rats ex vivo. Once these conditions
are established, we will use CCM to detect neuropathy in eyes of control and
diabetic mice provided from new models of diabetes developed by the AMDCC. We
will also compare the structural neuropathy detected by CCM with the current
methods of phenotyping neuropathy in tissues from these animals which are
reduced axonal calibre in the sciatic nerve and loss of intraepidermal nerve
fibers in the paw skin. The sensitivity of CCM to detect onset and progression
of neuropathy in a longitudinal study of the eyes of mouse models of type 1 and
type 2 diabetes will also be studied and compared with onset and progression of
nerve conduction velocity slowing and paw thermal hypoalgesia. The purpose of
these studies is to establish the viability of CCM as a sensitive means of
detecting neuropathy in diabetic rodents so that it can be used to phenotype new
models of diabetes being developed by the AMDCC and to allow assessment of the
efficacy of new therapeutics in rodent models prior to clinical trials using the
same end point and equipment.
Application PDF Application Research Plan
Status Contract Executed
Key Personnel
Salary Total Costs 5827
Supply Total Costs 0
Equipment Total Costs 47618
Travel/Other Total Costs 2176
Direct Costs 55621
Indirect Costs Proposed 4361
Total Costs Proposed 59982
Total Costs Approved 63000
Start Date 9/30/2009
End Date 8/31/2011
IFO Name Molina, Jan
IFO E-Mail Address jmolina@ucsd.edu
IACUC/IRB No. A3033-01
IACUC/IRB Institution University of California San Diego
Entity ID No. 1956006144A1
Report Request Date 9/30/2010
T1D NO
TypeCount
Invoices 0
Progress Reports 1
Experiments 1
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