¿´Æ¬ÊÓƵ

Object moved to here.

¿´Æ¬ÊÓƵ :: Pilot & Feasibility Program Application

¿´Æ¬ÊÓƵ


Microneurography in diabetic mice
Summary Data Summary
Applicant Baumann, Thomas
E-Mail Address tbaumann@legacyhealthresearch.org
Project Title Microneurography in diabetic mice
CBU ID 09MCG81
External SubContract ID 20497-32
Diabetic Complication Neuropathy & Neurocognition
Funding Program Group Pilot & Feasibility [PF2009]
Abstract Spontaneous burning pain is a common symptom of diabetic peripheral neuropathy
in humans. Yet there are few objective measures and no clear surrogate marker
for spontaneous pain in laboratory animals. The conventional
electrophysiological test currently employed by the consortium (nerve conduction
velocity measurement) is incapable of providing information regarding burning
pain because this test measures only the amplitude and latency of the compound
action potential produced by the large diameter (Aß) primary afferent fibers
which supply low-threshold mechanoreceptors. Unlike the small-diameter (C)
nociceptive fibers, Aß fibers do not contribute to the burning pain of diabetic
neuropathy. Small fiber neuropathy can exist in the absence of large fiber
neuropathy. Behavioral assays (measurement of withdrawal latencies in response
to noxious thermal stimulation) provide only an indirect measure of diabetic
neuropathy. Histologic assays (measurement of nerve fiber density in the skin)
cannot substitute for direct measurements of nerve fiber discharge. For these
reasons we propose to add microneurography to the arsenal employed for
phenotyping diabetic mice. Microneurography has been used for decades in studies
of the neural substrate of human somatosensory perception. There is a wealth of
background information. Among other achievements, microneurographic studies in
humans demonstrated that spontaneous burning pain in patients with diabetic
neuropathy is associated with spontaneous action potential discharge in
nociceptive C-fiber primary afferents. Recently the microneurography technique
was adapted for recording C-fiber activity in rats. We propose to adapt this
technique for use in mice. Comparison of the patterns of C-fiber discharge
measured in diabetic humans who do or do not experience spontaneous burning pain
to the patterns of the C-fiber activity measured in different strains of
diabetic mice will ultimately permit the selection of the most representative
mouse model of painful diabetic peripheral neuropathy.
Application PDF Application Research Plan
Status Contract Executed
Key Personnel
Salary Total Costs 28781
Supply Total Costs 10180
Equipment Total Costs 0
Travel/Other Total Costs 0
Direct Costs 38961
Indirect Costs Proposed 21039
Total Costs Proposed 60000
Total Costs Approved 63000
Start Date 9/1/2009
End Date 8/31/2010
IFO Name Malain, Cynthia
IFO E-Mail Address phillcyn@ohsu.edu
IACUC/IRB No. B11022
IACUC/IRB Institution Oregon Health Sciences University
Entity ID No. 1931176109A1
Report Request Date 9/30/2010
T1D NO
TypeCount
Invoices 0
Progress Reports 1
Experiments 1
Data Submission


Reports


Experiments
Click here to cancel and return to funding program application

*Author:
*SubContract:
*Select File:

Click browse and select the file to upload.
(Please upload ONLY TXT, Image Files (not histolgy), PDF, XLS, XLSX, DOC, or DOCX files.)